Human SERPINA3(Alpha-1-antichymotrypsin) ELISA Kit

Manual
  • Specifications
Product name

Human SERPINA3(Alpha-1-antichymotrypsin) ELISA Kit

Catalogue No.

EH0570

Alias

SERPINA3 ELISA Kit, ACT ELISA Kit, ACTGIG25 ELISA Kit, alpha-1-antichymotrypsin ELISA Kit, antitrypsin), member 3 ELISA Kit, Cell growth-inhibiting gene 24 ELISA Kit, 25 protein ELISA Kit, growth-inhibiting protein 24 ELISA Kit, growth-inhibiting protein 25 ELISA Kit, member 3 ELISA Kit, serine(or cysteine) proteinase inhibitor, clade A(alpha-1 antiproteinase ELISA Kit, serine(or cysteine) proteinase inhibitor, clade A, member 3 ELISA Kit, Serpin A3 ELISA Kit, serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SERPINA3 ELISA Kit allows for the in vitro quantitative determination of SERPINA3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

1.56-100nmol/ml

Sensitivity

< 0.938nmol/ml

Standard curve

Human SERPINA3(Alpha-1-antichymotrypsin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SERPINA3 and the recovery rates were calculated by comparing the measured value to the expected amount of SERPINA3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-102 97
EDTA plasma(n=5) 89-102 94
heparin plasma(n=5) 90-105 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SERPINA3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 85-102% 92-101% 92-105%
EDTA plasma(n=5) 86-98% 86-100% 89-100% 84-94%
heparin plasma(n=5) 83-90% 88-97% 85-95% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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