Human Rantes(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit

Manual
  • Specifications
Product name

Human Rantes(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit

Catalogue No.

EH0265

Alias

Rantes(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit, CCL5 ELISA Kit, SCYA5 ELISA Kit, SISd ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

Rantes ELISA Kit allows for the in vitro quantitative determination of Rantes concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human Rantes(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Rantes and the recovery rates were calculated by comparing the measured value to the expected amount of Rantes in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 93
EDTA plasma(n=5) 85-102 96
heparin plasma(n=5) 88-94 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rantes and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-95% 85-99% 87-103% 95-104%
EDTA plasma(n=5) 86-101% 85-100% 89-96% 83-95%
heparin plasma(n=5) 81-97% 82-99% 88-98% 86-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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