Human PI3(Peptidase Inhibitor 3, Skin Derived) ELISA Kit

Manual
  • Specifications
Product name

Human PI3(Peptidase Inhibitor 3, Skin Derived) ELISA Kit

Catalogue No.

EH0118

Alias

PI3 ELISA Kit, Elafin(Peptidase Inhibitor 3, Skin Derived) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PI3 ELISA Kit allows for the in vitro quantitative determination of PI3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human PI3(Peptidase Inhibitor 3, Skin Derived) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PI3 and the recovery rates were calculated by comparing the measured value to the expected amount of PI3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-103 99
EDTA plasma(n=5) 86-104 94
heparin plasma(n=5) 87-102 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PI3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 87-104% 87-105% 87-105%
EDTA plasma(n=5) 83-101% 83-99% 84-92% 88-100%
heparin plasma(n=5) 82-92% 82-96% 80-98% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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