Human PCSK9(Proprotein convertase subtilisin/kexin type 9) ELISA Kit

Manual
  • Specifications
Product name

Human PCSK9(Proprotein convertase subtilisin/kexin type 9) ELISA Kit

Catalogue No.

EH0251

Alias

PCSK9(Proprotein Convertase Subtilisin ELISA Kit, Kexin Type 9) ELISA Kit, Proprotein Convertase 9 ELISA Kit, FH3 ELISA Kit, HCHOLA3 ELISA Kit, NARC-1 ELISA Kit, PC9 ELISA Kit, HCHOLA3 ELISA Kit, LDLCQ1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PCSK9 ELISA Kit allows for the in vitro quantitative determination of PCSK9 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human PCSK9(Proprotein convertase subtilisin/kexin type 9) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PCSK9 and the recovery rates were calculated by comparing the measured value to the expected amount of PCSK9 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-105 100
EDTA plasma(n=5) 86-104 92
heparin plasma(n=5) 88-105 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCSK9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 87-101% 87-104% 90-98%
EDTA plasma(n=5) 84-95% 87-99% 91-101% 86-100%
heparin plasma(n=5) 83-99% 83-96% 80-99% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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