Human OPG(Osteoprotegerin) ELISA Kit

Manual
  • Specifications
Product name

Human OPG(Osteoprotegerin) ELISA Kit

Catalogue No.

EH0247

Alias

OPG(Osteoprotegerin) ELISA Kit, OCIF ELISA Kit, TNFRSF11B ELISA Kit, OPGtumor necrosis factor receptor superfamily member 11B ELISA Kit, Osteoclastogenesis inhibitory factor ELISA Kit, Osteoprotegerin ELISA Kit, TR1 ELISA Kit, tumor necrosis factor receptor superfamily, member 11b ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

OPG ELISA Kit allows for the in vitro quantitative determination of OPG concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human OPG(Osteoprotegerin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of OPG and the recovery rates were calculated by comparing the measured value to the expected amount of OPG in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-105 97
EDTA plasma(n=5) 88-99 94
heparin plasma(n=5) 94-101 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 85-96% 88-105% 92-105%
EDTA plasma(n=5) 82-91% 92-100% 83-97% 84-99%
heparin plasma(n=5) 82-99% 82-100% 80-100% 82-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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