Human NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Manual
  • Specifications
Product name

Human NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Catalogue No.

EH0350

Alias

NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NT-proBNP ELISA Kit allows for the in vitro quantitative determination of NT-proBNP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

39.063-2500pg/ml

Sensitivity

< 23.438pg/ml

Standard curve

Human NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NT-proBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-proBNP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 92
EDTA plasma(n=5) 90-104 98
heparin plasma(n=5) 93-100 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-proBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-101% 85-105% 85-105% 92-105%
EDTA plasma(n=5) 85-99% 84-98% 88-100% 86-101%
heparin plasma(n=5) 81-99% 81-88% 86-100% 80-93%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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