Human NT-3(Neurotrophin-3) ELISA Kit

Manual
  • Specifications
Product name

Human NT-3(Neurotrophin-3) ELISA Kit

Catalogue No.

EH0245

Alias

NT-3(Neurotrophin-3) ELISA Kit, NTF3 ELISA Kit, 3-nucleotidase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NT-3 ELISA Kit allows for the in vitro quantitative determination of NT-3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human NT-3(Neurotrophin-3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NT-3 and the recovery rates were calculated by comparing the measured value to the expected amount of NT-3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-98 91
EDTA plasma(n=5) 93-105 100
heparin plasma(n=5) 86-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 87-101% 85-102% 92-104%
EDTA plasma(n=5) 90-101% 83-97% 83-101% 82-101%
heparin plasma(n=5) 82-97% 86-99% 81-100% 85-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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