Human NPPC(C-type natriuretic peptide) ELISA Kit

Manual
  • Specifications
Product name

Human NPPC(C-type natriuretic peptide) ELISA Kit

Catalogue No.

EH1023

Alias

NPPC ELISA Kit, CNP ELISA Kit, CNP2 ELISA Kit, C-type natriuretic peptide ELISA Kit, natriuretic peptide C ELISA Kit, natriuretic peptide precursor C ELISA Kit

Detection method

Competitive ELISA, Coated with Antigen

Application

NPPC ELISA Kit allows for the in vitro quantitative determination of NPPC concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human NPPC(C-type natriuretic peptide) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NPPC and the recovery rates were calculated by comparing the measured value to the expected amount of NPPC in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-99 94
EDTA plasma(n=5) 90-103 99
heparin plasma(n=5) 86-96 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NPPC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 87-96% 88-105% 87-105%
EDTA plasma(n=5) 85-99% 85-101% 84-97% 92-100%
heparin plasma(n=5) 80-99% 88-97% 81-100% 84-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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