Human NNE(Non-Neuronal Enolase) ELISA Kit

Manual
  • Specifications
Product name

Human NNE(Non-Neuronal Enolase) ELISA Kit

Catalogue No.

EH0371

Alias

NNE(Non-neural enolase) ELISA Kit, ENO1 ELISA Kit, Alpha-enolase ELISA Kit, MPB-1 ELISA Kit, MBP-1 ELISA Kit, Enolase 1 ELISA Kit, Plasminogen-binding protein ELISA Kit, Phosphopyruvate hydratase ELISA Kit, 2-phospho-D-glycerate hydro-lyase ELISA Kit, C-myc promoter-binding protein ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NNE ELISA Kit allows for the in vitro quantitative determination of NNE concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human NNE(Non-Neuronal Enolase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NNE and the recovery rates were calculated by comparing the measured value to the expected amount of NNE in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-99 94
EDTA plasma(n=5) 85-104 95
heparin plasma(n=5) 87-103 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-103% 85-103% 91-105% 88-96%
EDTA plasma(n=5) 87-97% 83-99% 86-96% 82-90%
heparin plasma(n=5) 84-93% 85-98% 81-92% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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