Human NID-1(Nidogen-1/Entactin) ELISA Kit

Manual
  • Specifications
Product name

Human NID-1(Nidogen-1/Entactin) ELISA Kit

Catalogue No.

EH0243

Alias

Nidogen-1 ELISA Kit, Entactin ELISA Kit, NID-1 ELISA Kit, Entactin-1 ELISA Kit, NID1 ELISA Kit, NIDentactin ELISA Kit, nidogen(enactin) ELISA Kit, nidogen 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NID-1 ELISA Kit allows for the in vitro quantitative determination of NID-1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human NID-1(Nidogen-1/Entactin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NID-1 and the recovery rates were calculated by comparing the measured value to the expected amount of NID-1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-100 96
EDTA plasma(n=5) 89-105 100
heparin plasma(n=5) 87-104 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NID-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 88-103% 88-104% 86-104%
EDTA plasma(n=5) 82-100% 84-101% 85-98% 83-95%
heparin plasma(n=5) 82-99% 82-99% 81-99% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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