Human NGF/NGFβ(Beta-nerve growth factor) ELISA Kit

Manual
  • Specifications
Product name

Human NGF/NGFβ(Beta-nerve growth factor) ELISA Kit

Catalogue No.

EH0242

Alias

NGF ELISA Kit, NGFβ ELISA Kit, NGFB ELISA Kit, beta-nerve growth factor ELISA Kit, Beta-NGF ELISA Kit, HSAN5 ELISA Kit, nerve growth factor(beta polypeptide) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NGF/NGFβ ELISA Kit allows for the in vitro quantitative determination of NGF/NGFβ concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human NGF/NGFβ(Beta-nerve growth factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NGF/NGFβ and the recovery rates were calculated by comparing the measured value to the expected amount of NGF/NGFβ in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 94
EDTA plasma(n=5) 89-105 96
heparin plasma(n=5) 90-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF/NGFβ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 85-103% 88-105% 88-105%
EDTA plasma(n=5) 88-101% 85-97% 91-99% 85-101%
heparin plasma(n=5) 81-98% 88-97% 88-100% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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