Human MUC5B(Mucin-5B) ELISA Kit

Manual
  • Specifications
Product name

Human MUC5B(Mucin-5B) ELISA Kit

Catalogue No.

EH1005

Alias

MUC5B ELISA Kit, MUC5 ELISA Kit, MUC-5B ELISA Kit, Sublingual gland mucin ELISA Kit, Mucin-5 subtype B, tracheobronchial ELISA Kit, Cervical mucin ELISA Kit, High molecular weight salivary mucin MG1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MUC5B ELISA Kit allows for the in vitro quantitative determination of MUC5B concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human MUC5B(Mucin-5B) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MUC5B and the recovery rates were calculated by comparing the measured value to the expected amount of MUC5B in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-103 97
EDTA plasma(n=5) 90-102 96
heparin plasma(n=5) 89-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MUC5B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 87-99% 87-105% 86-104%
EDTA plasma(n=5) 88-98% 83-101% 87-94% 84-100%
heparin plasma(n=5) 80-94% 80-100% 86-100% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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