Human MMP13(Matrix Metalloproteinase 13) ELISA Kit

Manual
  • Specifications
Product name

Human MMP13(Matrix Metalloproteinase 13) ELISA Kit

Catalogue No.

EH0234

Alias

MMP-13(Matrix Metalloproteinase 13) ELISA Kit, MMP-13 ELISA Kit, CLG3 ELISA Kit, MANDP1 ELISA Kit, Collagenase 3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MMP13 ELISA Kit allows for the in vitro quantitative determination of MMP13 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human MMP13(Matrix Metalloproteinase 13) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MMP13 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP13 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 92
EDTA plasma(n=5) 86-104 98
heparin plasma(n=5) 88-103 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 87-100% 89-105% 86-105%
EDTA plasma(n=5) 84-100% 82-100% 88-94% 82-101%
heparin plasma(n=5) 86-96% 85-93% 83-100% 83-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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