Human MMP-8(Matrix Metalloproteinase 8) ELISA Kit

Manual
  • Specifications
Product name

Human MMP-8(Matrix Metalloproteinase 8) ELISA Kit

Catalogue No.

EH0237

Alias

MMP-8(Matrix Metalloproteinase 8) ELISA Kit, Collagenase 2 ELISA Kit, CLG1HNC ELISA Kit, neutrophil collagenase ELISA Kit, PMNL collagenase ELISA Kit, PMNL-CL ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MMP-8 ELISA Kit allows for the in vitro quantitative determination of MMP-8 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human MMP-8(Matrix Metalloproteinase 8) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MMP-8 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-8 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-98 92
EDTA plasma(n=5) 96-102 99
heparin plasma(n=5) 87-99 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-104% 91-101% 91-105% 87-101%
EDTA plasma(n=5) 84-97% 85-92% 82-100% 83-91%
heparin plasma(n=5) 81-99% 83-100% 83-99% 81-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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