Human MIA(Melanoma Inhibitory Activity Protein 1) ELISA Kit

Manual
  • Specifications
Product name

Human MIA(Melanoma Inhibitory Activity Protein 1) ELISA Kit

Catalogue No.

EH0227

Alias

MIA1 ELISA Kit, MIA ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MIA ELISA Kit allows for the in vitro quantitative determination of MIA concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.813-500pg/ml

Sensitivity

< 4.688pg/ml

Standard curve

Human MIA(Melanoma Inhibitory Activity Protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MIA and the recovery rates were calculated by comparing the measured value to the expected amount of MIA in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-100 98
EDTA plasma(n=5) 87-103 95
heparin plasma(n=5) 94-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-99% 89-97% 87-95% 87-95%
EDTA plasma(n=5) 86-100% 82-95% 89-101% 91-97%
heparin plasma(n=5) 80-93% 81-100% 81-99% 84-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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