Human KITLG(Kit ligand ) ELISA Kit

Manual
  • Specifications
Product name

Human KITLG(Kit ligand ) ELISA Kit

Catalogue No.

EH0514

Alias

SCF(Stem Cell Factor) ELISA Kit, KITLG ELISA Kit, Kit ligand ELISA Kit, c-Kit ligand ELISA Kit, MGF ELISA Kit, Mast cell growth factor ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

KITLG ELISA Kit allows for the in vitro quantitative determination of KITLG concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human KITLG(Kit ligand ) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of KITLG and the recovery rates were calculated by comparing the measured value to the expected amount of KITLG in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-102 96
EDTA plasma(n=5) 89-102 98
heparin plasma(n=5) 89-101 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KITLG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 85-97% 86-103% 87-102%
EDTA plasma(n=5) 82-94% 88-96% 84-95% 83-98%
heparin plasma(n=5) 91-100% 84-99% 80-97% 80-92%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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