Human IL-6(Interleukin 6) ELISA Kit

Manual
  • Specifications
Product name

Human IL-6(Interleukin 6) ELISA Kit

Catalogue No.

EH0201

Alias

IL-6(Interleukin 6) ELISA Kit, IL6 ELISA Kit, BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, CDF ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

4.69-300pg/ml

Sensitivity

< 2.813pg/ml

Standard curve

Human IL-6(Interleukin 6) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-101 96
EDTA plasma(n=5) 92-103 96
heparin plasma(n=5) 89-101 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-102% 85-105% 86-105% 85-98%
EDTA plasma(n=5) 86-97% 92-100% 87-101% 92-101%
heparin plasma(n=5) 87-96% 94-100% 87-91% 83-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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