Human IL-3(Interleukin 3) ELISA Kit

Manual
  • Specifications
Product name

Human IL-3(Interleukin 3) ELISA Kit

Catalogue No.

EH0196

Alias

IL-3 ELISA Kit, IL3 ELISA Kit, MCGF ELISA Kit, MCSF ELISA Kit, MULTI-CSF ELISA Kit, P-Cell Stimulating Factor ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-3 ELISA Kit allows for the in vitro quantitative determination of IL-3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human IL-3(Interleukin 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-3 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 92-102 98
EDTA plasma(n=5) 88-98 93
heparin plasma(n=5) 85-99 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-99% 87-102% 94-101% 85-105%
EDTA plasma(n=5) 82-100% 92-100% 89-99% 84-98%
heparin plasma(n=5) 92-100% 83-96% 81-96% 87-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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