Human IL-2(Interleukin 2) ELISA Kit

Manual
  • Specifications
Product name

Human IL-2(Interleukin 2) ELISA Kit

Catalogue No.

EH0189

Alias

IL-2(Interleukin 2) ELISA Kit, IL2 ELISA Kit, TCGF ELISA Kit, Lymphokine ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-2 ELISA Kit allows for the in vitro quantitative determination of IL-2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human IL-2(Interleukin 2) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-2 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-101 94
EDTA plasma(n=5) 88-98 92
heparin plasma(n=5) 90-101 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-101% 86-101% 85-105% 87-103%
EDTA plasma(n=5) 94-100% 88-100% 86-94% 84-100%
heparin plasma(n=5) 84-98% 82-90% 82-97% 85-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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