Human IL-27(Interleukin-27 subunit alpha) ELISA Kit

Manual
  • Specifications
Product name

Human IL-27(Interleukin-27 subunit alpha) ELISA Kit

Catalogue No.

EH0193

Alias

IL-27(IL-27 subunit alpha) ELISA Kit, IL-27-A ELISA Kit, IL27-A ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-27 ELISA Kit allows for the in vitro quantitative determination of IL-27 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human IL-27(Interleukin-27 subunit alpha) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-27 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-27 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-101 93
EDTA plasma(n=5) 90-105 97
heparin plasma(n=5) 95-100 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 94-102% 86-105% 86-105%
EDTA plasma(n=5) 88-96% 82-101% 85-98% 85-101%
heparin plasma(n=5) 89-99% 82-93% 84-100% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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