Human IFN-β (Interferon beta ) ELISA Kit

Manual
  • Specifications
Product name

Human IFN-β (Interferon beta ) ELISA Kit

Catalogue No.

EH0485

Alias

IFN-β ELISA Kit, IFNB1 ELISA Kit, Fibroblast interferon ELISA Kit, IFBIFNB ELISA Kit, IFF ELISA Kit, IFN-beta ELISA Kit, interferon beta ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IFN-β ELISA Kit allows for the in vitro quantitative determination of IFN-β concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human IFN-β (Interferon beta ) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IFN-β and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-β in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 97
EDTA plasma(n=5) 87-103 96
heparin plasma(n=5) 86-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 88-94% 89-104% 91-95%
EDTA plasma(n=5) 83-100% 84-93% 88-101% 86-101%
heparin plasma(n=5) 81-96% 80-99% 82-89% 81-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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