Human IDH1(Isocitrate dehydrogenase [NADP] cytoplasmic) ELISA Kit

Manual
  • Specifications
Product name

Human IDH1(Isocitrate dehydrogenase [NADP] cytoplasmic) ELISA Kit

Catalogue No.

EH1337

Alias

IDH1 ELISA Kit, Isocitrate dehydrogenase [NADP] cytoplasmic ELISA Kit, Oxalosuccinate decarboxylase ELISA Kit, NADP(+)-specific ICDH ELISA Kit, IDH ELISA Kit, Cytosolic NADP-isocitrate dehydrogenase ELISA Kit, IDP ELISA Kit, PICD ELISA Kit, IDCD ELISA Kit, IDPC ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IDH1 ELISA Kit allows for the in vitro quantitative determination of IDH1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.78-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human IDH1(Isocitrate dehydrogenase [NADP] cytoplasmic) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IDH1 and the recovery rates were calculated by comparing the measured value to the expected amount of IDH1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-100 93
EDTA plasma(n=5) 95-104 99
heparin plasma(n=5) 87-103 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IDH1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-102% 89-101% 88-105% 90-96%
EDTA plasma(n=5) 88-101% 87-101% 88-96% 83-97%
heparin plasma(n=5) 80-97% 86-98% 85-94% 89-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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