Human HAS2(Hyaluronan synthase 2) ELISA Kit

Manual
  • Specifications
Product name

Human HAS2(Hyaluronan synthase 2) ELISA Kit

Catalogue No.

EH1302

Alias

HAS2(Hyaluronan synthase 2) ELISA Kit, HA synthase 2 ELISA Kit, Hyaluronate synthase 2 ELISA Kit, Hyaluronic acid synthase 2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

HAS2 ELISA Kit allows for the in vitro quantitative determination of HAS2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human HAS2(Hyaluronan synthase 2) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HAS2 and the recovery rates were calculated by comparing the measured value to the expected amount of HAS2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-102 93
EDTA plasma(n=5) 87-104 100
heparin plasma(n=5) 87-96 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HAS2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-99% 91-104% 87-105% 86-103%
EDTA plasma(n=5) 84-94% 88-98% 82-100% 89-97%
heparin plasma(n=5) 83-100% 84-99% 88-89% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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