Human GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit

Manual
  • Specifications
Product name

Human GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit

Catalogue No.

EH0153

Alias

GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit, GMCSF ELISA Kit, CSF2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

GM-CSF ELISA Kit allows for the in vitro quantitative determination of GM-CSF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.813-500pg/ml

Sensitivity

< 4.688pg/ml

Standard curve

Human GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GM-CSF and the recovery rates were calculated by comparing the measured value to the expected amount of GM-CSF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-103 92
EDTA plasma(n=5) 93-100 96
heparin plasma(n=5) 87-103 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GM-CSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 86-97% 92-104% 86-105%
EDTA plasma(n=5) 83-99% 87-101% 82-97% 83-100%
heparin plasma(n=5) 85-94% 84-96% 87-100% 81-91%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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