Human GCK(Glucokinase) ELISA Kit

Manual
  • Specifications
Product name

Human GCK(Glucokinase) ELISA Kit

Catalogue No.

EH0997

Alias

GCK(Glucokinase) ELISA Kit, FGQTL3 ELISA Kit, GLK ELISA Kit, Hexokinase-4 ELISA Kit, Hexokinase-D ELISA Kit, HHF3 ELISA Kit, HK4 ELISA Kit, HXKP ELISA Kit, LGLK ELISA Kit, GK ELISA Kit, glucokinase(hexokinase 4) ELISA Kit, HKIV ELISA Kit, maturity onset diabetes of the young 2 ELISA Kit, MODY2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

GCK ELISA Kit allows for the in vitro quantitative determination of GCK concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human GCK(Glucokinase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GCK and the recovery rates were calculated by comparing the measured value to the expected amount of GCK in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-101 95
EDTA plasma(n=5) 86-99 92
heparin plasma(n=5) 86-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 85-104% 87-104% 90-104%
EDTA plasma(n=5) 82-98% 88-100% 83-97% 88-99%
heparin plasma(n=5) 83-100% 89-100% 82-100% 81-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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