Human COL3A1(Collagen alpha-1(III) chain) ELISA Kit

Manual
  • Specifications
Product name

Human COL3A1(Collagen alpha-1(III) chain) ELISA Kit

Catalogue No.

EH0746

Alias

COL3α1(Collagen Type III Alpha 1) ELISA Kit, COL3A1 ELISA Kit, EDS4A ELISA Kit, alpha1(III) collagen ELISA Kit, Collagen 3 ELISA Kit, collagen alpha-1(III) chain ELISA Kit, collagen, fetal ELISA Kit, collagen, type III, alpha 1 ELISA Kit, Collagen-3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

COL3A1 chain) ELISA Kit allows for the in vitro quantitative determination of COL3A1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human COL3A1(Collagen alpha-1(III) chain) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of COL3A1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL3A1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-95 90
EDTA plasma(n=5) 91-100 97
heparin plasma(n=5) 85-103 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL3A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 85-94% 90-105% 88-103%
EDTA plasma(n=5) 87-101% 87-101% 94-96% 91-101%
heparin plasma(n=5) 82-97% 92-100% 82-99% 82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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