Human CD86(T-lymphocyte activation antigen CD86) ELISA Kit

Manual
  • Specifications
Product name

Human CD86(T-lymphocyte activation antigen CD86) ELISA Kit

Catalogue No.

EH0659

Alias

B7-2 ELISA Kit, CD86 ELISA Kit, Activation B7-2 antigen ELISA Kit, B70 ELISA Kit, B7-2 antigen ELISA Kit, B-lymphocyte activation antigen B7-2 ELISA Kit, BU63 ELISA Kit, CD28 antigen ligand 2 ELISA Kit, CD28LG2 ELISA Kit, CD86 antigen ELISA Kit, CD86 molecule ELISA Kit, CTLA-4 counter-receptor B7.2 ELISA Kit, FUN-1 ELISA Kit, LAB72 ELISA Kit, T-lymphocyte activation antigen CD86 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CD86 ELISA Kit allows for the in vitro quantitative determination of CD86 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human CD86(T-lymphocyte activation antigen CD86) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CD86 and the recovery rates were calculated by comparing the measured value to the expected amount of CD86 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-100 92
EDTA plasma(n=5) 93-104 98
heparin plasma(n=5) 89-104 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD86 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 86-101% 90-105% 87-105%
EDTA plasma(n=5) 83-100% 85-94% 89-95% 83-99%
heparin plasma(n=5) 81-97% 84-94% 81-97% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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