Human CCL4(C-C motif chemokine 4) ELISA Kit

Manual
  • Specifications
Product name

Human CCL4(C-C motif chemokine 4) ELISA Kit

Catalogue No.

EH0067

Alias

CCL4 ELISA Kit, MIP-1β ELISA Kit, ACT2 ELISA Kit, AT744.1 ELISA Kit, G-26 ELISA Kit, HC21 ELISA Kit, LAG-1 ELISA Kit, LAG1 ELISA Kit, MIP-1-beta ELISA Kit, MIP1B1 ELISA Kit, SCYA2 ELISA Kit, SCYA4 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CCL4 ELISA Kit allows for the in vitro quantitative determination of CCL4 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human CCL4(C-C motif chemokine 4) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CCL4 and the recovery rates were calculated by comparing the measured value to the expected amount of CCL4 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-103 97
EDTA plasma(n=5) 88-105 94
heparin plasma(n=5) 88-94 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 85-104% 85-101% 86-105%
EDTA plasma(n=5) 87-94% 85-99% 82-96% 89-100%
heparin plasma(n=5) 80-100% 86-99% 91-100% 85-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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