Human CA4(Carbonic anhydrase 4) ELISA Kit

Manual
  • Specifications
Product name

Human CA4(Carbonic anhydrase 4) ELISA Kit

Catalogue No.

EH1257

Alias

CA4(Carbonic Anhydrase IV) ELISA Kit, CAIV ELISA Kit, CA-IV ELISA Kit, Car4 ELISA Kit, RP17 ELISA Kit, Carbonate dehydratase IV ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CA4 ELISA Kit allows for the in vitro quantitative determination of CA4 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human CA4(Carbonic anhydrase 4) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CA4 and the recovery rates were calculated by comparing the measured value to the expected amount of CA4 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-105 98
EDTA plasma(n=5) 87-93 90
heparin plasma(n=5) 89-102 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 91-101% 95-103% 89-100%
EDTA plasma(n=5) 83-94% 82-101% 83-95% 85-97%
heparin plasma(n=5) 80-94% 82-98% 80-95% 85-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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