Human CA19-9(Carbohydrate antigen19-9) ELISA Kit

Manual
  • Specifications
Product name

Human CA19-9(Carbohydrate antigen19-9) ELISA Kit

Catalogue No.

EH0363

Alias

CA19-9(Carbohydrate antigen19-9) ELISA Kit, CA199 ELISA Kit, sialylated Lewis(a)Antigen ELISA Kit, Cancer Antigen 19-9 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CA19-9 ELISA Kit allows for the in vitro quantitative determination of CA19-9 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.125-200IU/ml

Sensitivity

< 1.875U/ml

Standard curve

Human CA19-9(Carbohydrate antigen19-9) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CA19-9 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-9 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-104 93
EDTA plasma(n=5) 88-104 98
heparin plasma(n=5) 94-98 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 95-104% 85-102% 86-100%
EDTA plasma(n=5) 87-98% 85-99% 84-100% 82-101%
heparin plasma(n=5) 83-100% 81-98% 80-95% 82-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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