Human C4A(Complement C4-A) ELISA Kit

Manual
  • Specifications
Product name

Human C4A(Complement C4-A) ELISA Kit

Catalogue No.

EH0646

Alias

C4A ELISA Kit, C4AD ELISA Kit, C4S ELISA Kit, CO4 ELISA Kit, CPAMD2 ELISA Kit, RG ELISA Kit, Complement Component 4a ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

C4A ELISA Kit allows for the in vitro quantitative determination of C4A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

1.563-100ng/ml

Sensitivity

< 0.938ng/ml

Standard curve

Human C4A(Complement C4-A) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of C4A and the recovery rates were calculated by comparing the measured value to the expected amount of C4A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 93
EDTA plasma(n=5) 89-97 94
heparin plasma(n=5) 91-102 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-99% 85-104% 86-103% 90-103%
EDTA plasma(n=5) 84-101% 83-99% 83-100% 90-101%
heparin plasma(n=5) 80-95% 80-89% 85-100% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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