Human BMP-5(Bone Morphogenetic Protein 5) ELISA Kit

Manual
  • Specifications
Product name

Human BMP-5(Bone Morphogenetic Protein 5) ELISA Kit

Catalogue No.

EH0047

Alias

BMP-5(Bone Morphogenetic Protein 5) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

BMP-5 ELISA Kit allows for the in vitro quantitative determination of BMP-5 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human BMP-5(Bone Morphogenetic Protein 5) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BMP-5 and the recovery rates were calculated by comparing the measured value to the expected amount of BMP-5 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 92-101 97
EDTA plasma(n=5) 92-104 97
heparin plasma(n=5) 87-105 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMP-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 86-104% 85-101% 94-103%
EDTA plasma(n=5) 88-99% 83-99% 86-98% 85-100%
heparin plasma(n=5) 87-97% 87-100% 81-100% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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