Human Bcl-2(B-cell Leukemia/Lymphoma 2) ELISA Kit

Manual
  • Specifications
Product name

Human Bcl-2(B-cell Leukemia/Lymphoma 2) ELISA Kit

Catalogue No.

EH0658

Alias

Bcl-2 ELISA Kit, apoptosis regulator Bcl-2 ELISA Kit, B-cell CLL ELISA Kit, lymphoma 2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

Bcl-2 ELISA Kit allows for the in vitro quantitative determination of Bcl-2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human Bcl-2(B-cell Leukemia/Lymphoma 2) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Bcl-2 and the recovery rates were calculated by comparing the measured value to the expected amount of Bcl-2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-96 91
EDTA plasma(n=5) 85-101 92
heparin plasma(n=5) 90-105 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bcl-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-103% 91-105% 86-98% 89-102%
EDTA plasma(n=5) 88-96% 83-101% 83-96% 82-101%
heparin plasma(n=5) 84-93% 82-93% 86-95% 83-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

点击这里给我发消息

Hotline

027-87384275