Human B3GALT1(Beta-1,3-galactosyltransferase 1) ELISA Kit

Manual
  • Specifications
Product name

Human B3GALT1(Beta-1,3-galactosyltransferase 1) ELISA Kit

Catalogue No.

EH1329

Alias

B3GALT1 ELISA Kit, Beta-1,3-galactosyltransferase 1 ELISA Kit, Beta-1,3-GalTase 1 ELISA Kit, Beta3Gal-T1 ELISA Kit, Beta3GalT1 ELISA Kit, UDP-galactose:beta-N-acetyl-glucosamine-beta-1,3-galactosyltransferase 1 ELISA Kit

Detection method

Competitive ELISA, Coated with Antibody

Application

B3GALT1 ELISA Kit allows for the in vitro quantitative determination of B3GALT1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human B3GALT1(Beta-1,3-galactosyltransferase 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of B3GALT1 and the recovery rates were calculated by comparing the measured value to the expected amount of B3GALT1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-102 95
EDTA plasma(n=5) 88-103 97
heparin plasma(n=5) 91-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of B3GALT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 85-95% 88-102% 85-102%
EDTA plasma(n=5) 82-100% 82-101% 83-101% 84-97%
heparin plasma(n=5) 80-99% 80-87% 80-99% 84-90%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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