Human APP(Amyloid beta A4 protein) ELISA Kit

Manual
  • Specifications
Product name

Human APP(Amyloid beta A4 protein) ELISA Kit

Catalogue No.

EH0039

Alias

APP(Amyloid Precursor Protein) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

APP ELISA Kit allows for the in vitro quantitative determination of APP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve

Human APP(Amyloid beta A4 protein) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of APP and the recovery rates were calculated by comparing the measured value to the expected amount of APP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-102 94
EDTA plasma(n=5) 85-105 93
heparin plasma(n=5) 85-102 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 89-103% 87-103% 101-105%
EDTA plasma(n=5) 88-100% 83-99% 89-99% 85-100%
heparin plasma(n=5) 82-91% 83-97% 81-91% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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