Human AFP(alpha Fetoprotein) ELISA Kit

Manual
  • Specifications
Product name

Human AFP(alpha Fetoprotein) ELISA Kit

Catalogue No.

EH0359

Alias

αFP(Alpha-Fetoprotein) ELISA Kit, AFP ELISA Kit, FETA ELISA Kit, HPAFP ELISA Kit, oralpha fetal protein ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

AFP ELISA Kit allows for the in vitro quantitative determination of AFP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human AFP(alpha Fetoprotein) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of AFP and the recovery rates were calculated by comparing the measured value to the expected amount of AFP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 94
EDTA plasma(n=5) 85-103 93
heparin plasma(n=5) 86-97 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AFP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-105% 90-93% 85-98% 86-98%
EDTA plasma(n=5) 89-101% 84-94% 83-101% 86-101%
heparin plasma(n=5) 81-97% 85-96% 82-96% 84-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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