Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) ELISA Kit

Manual
  • Specifications
Product name

Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) ELISA Kit

Catalogue No.

EH0480

Alias

ACAT2(Acetyl Coenzyme A Acetyltransferase 2, cytosolic) ELISA Kit, cytosolic Acetoacetyl-CoA Thiolase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ACAT2 ELISA Kit allows for the in vitro quantitative determination of ACAT2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Standard curve

Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ACAT2 and the recovery rates were calculated by comparing the measured value to the expected amount of ACAT2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 93
EDTA plasma(n=5) 86-95 91
heparin plasma(n=5) 89-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACAT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 90-104% 86-94% 96-105%
EDTA plasma(n=5) 84-94% 87-99% 85-97% 84-100%
heparin plasma(n=5) 83-97% 82-95% 81-96% 81-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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