Human A-CoA (Acetyl Coenzyme A) ELISA Kit

Manual
  • Specifications
Product name

Human A-CoA (Acetyl Coenzyme A) ELISA Kit

Catalogue No.

EH0606

Alias

A-CoA(Acetyl Coenzyme A) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

A-CoA ELISA Kit allows for the in vitro quantitative determination of A-CoA concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human A-CoA (Acetyl Coenzyme A) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of A-CoA and the recovery rates were calculated by comparing the measured value to the expected amount of A-CoA in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 94
EDTA plasma(n=5) 88-102 93
heparin plasma(n=5) 86-104 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A-CoA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 88-99% 89-101% 90-105%
EDTA plasma(n=5) 82-93% 83-94% 88-98% 82-94%
heparin plasma(n=5) 87-98% 86-100% 84-100% 89-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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