Chicken HP(Haptoglobin) ELISA Kit

Manual
  • Specifications
Product name

Chicken HP(Haptoglobin) ELISA Kit

Catalogue No.

ECH0023

Detection method

Sandwich ELISA, Double Antibody

Application

HP ELISA Kit allows for the in vitro quantitative determination of HP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000ng/ml

Sensitivity

< 18.75ng/ml

Standard curve

Chicken HP(Haptoglobin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HP and the recovery rates were calculated by comparing the measured value to the expected amount of HP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-105 98
EDTA plasma(n=5) 86-105 96
heparin plasma(n=5) 87-105 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 85-105% 85-101% 89-105%
EDTA plasma(n=5) 84-92% 82-99% 82-97% 85-100%
heparin plasma(n=5) 87-95% 83-96% 87-97% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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