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ZO-1 antibody
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Synonyms: | Tight junction protein 1 antibody, Tight junction protein ZO 1 antibody, TJP1 antibody, ZO 1 antibody, ZO1 antibody, ZO-1 antibody, Zona occludens protein 1 antibody, Zonula occludens protein 1 antibody | ||
Catalogue No.: | FNab09751 | Reativity: | Human, Mouse, Rat |
Host: | Rabbit | Tested Application: | ELISA, WB, IHC, FC, IF, IP |
Clonality: | polyclonal | Isotype: | IgG |
- SPECIFICATIONS
- Product Name
- ZO-1 antibody
- Catalogue No.
- FNab09751
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tight junction protein 1(zona occludens 1)
- Alternative Names
- Tight junction protein 1 antibody, Tight junction protein ZO 1 antibody, TJP1 antibody, ZO 1 antibody, ZO1 antibody, ZO-1 antibody, Zona occludens protein 1 antibody, Zonula occludens protein 1 antibody
- UniProt ID
- Q07157
- Observed MW
- 230kd
Application
- Tested Applications
- ELISA, WB, IHC, FC, IF, IP
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09751(ZO1 antibody) at dilution of 1:500
IP result of anti-ZO-1(IP:FNab09751, 4ug; Detection:FNab09751 1:500) with HEK-293 cells lysate 2320 ug
Immunohistochemistry of paraffin-embedded human kidney using FNab09751(ZO1 antibody) at dilution of 1:100
Immunofluorescent analysis of MCF7 cells using FNab09751(ZO-1 Antibody) at dilution of 1:100
- Background
- Tight junction(or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium(PMID: 20066090). ZO-1(also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells(PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin(PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs(MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation(PMID: 22782886).