Products

DHFR antibody

Synonyms:DHFR antibody, DHFRP1 antibody, dihydrofolate reductase antibody
Catalogue No.:FNab02365Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
DHFR antibody
Catalogue No.
FNab02365
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
dihydrofolate reductase
Alternative Names
DHFR antibody, DHFRP1 antibody, dihydrofolate reductase antibody
UniProt ID
P00374
Observed MW
21 kDa
Application
Tested Applications
ELISA, WB, IHC, IP, IF
Recommended dilution
WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
K-562 cells were subjected to SDS PAGE followed by western blot with FNab02365(DHFR antibody) at dilution of 1:500
IP Result of anti-DHFR (IP: FNab02365, 3ug; Detection: FNab02365 1:500) with Raji cells lysate 2000ug
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab02365(DHFR Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using FNab02365 (DHFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.