Products
6*His, His-Tag antibody
- Product Name
- 6*His, His-Tag antibody
- Catalogue No.
- FNab00008
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 1C4
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- 6*His antibody, 6*His antibody, His Tag antibody, 6histag antibody, 6XHis antibody, HHHHHH antibody, HIS antibody, His Tag antibody, HISTIDINE TAGGED antibody
Application
- Tested Applications
- ELISA, WB, IP, IF
- Recommended dilution
- WB: 1:1000-1:10000; IP: 1:1000-1:10000; IF: 1:20-1:100
Validated Images
Western blot of 6*His-tagged fusion protein with FNab00008(anti-6*HIS tag) at dilution of 1:5000.
IP Result of anti-6*His, His-Tag (IP: FNab00008, 7ug; Detection: FNab00008 1:500) with Transfected HEK-293 cells lysate 300ug
Immunofluorescent analysis of Transfected HEK-293 cells using FNab00008 ( 6*His, His-Tag Antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
- Background
- Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
